a simple and sensitive hplc method for fluorescence quantitation of doxorubicin in micro-volume plasma: applications to pharmacokinetic studies in rats

نویسندگان

marjan daeihamed school of pharmacy, shahid beheshti university of medical sciences

azadeh haeri school of pharmacy, shahid beheshti university of medical sciences

simin dadashzadeh department of pharmaceutics, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran

چکیده

a validated hplc method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. the analysis was accomplished using perfectsil c18 column maintained at 35 °c and a mobile phase consisted of acetonitrile and water (32:68, v/v; ph=2.6). the flow-rate was kept at 1 ml/min and column effluent was monitored with a fluorescence detector at an excitation and emission wavelength of 470 and 555 nm, respectively. the detection limit was 5 ng/ml. no analytical interference was observed from endogenous components in rat plasma. this method was feasibly applied to the pharmacokinetic study of 5 mg/kg of doxorubicin after intravenous administration to rats.

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عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۴، شماره supplement، صفحات ۳۳-۴۲

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